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The C. elegans gustatory receptor homolog LITE-1 is a chemoreceptor required for diacetyl avoidance

Koh, A.; Bokman, E.; Gavrikov, A.; Rodriguez, J.; Chen, C.; Zaslaver, A.; Brown, A. E. X.

2025-04-24 neuroscience
10.1101/2025.04.20.649642 bioRxiv
Show abstract

The nematode C. elegans does not have eyes but can respond to aversive UV and blue light stimulation and even distinguish colours. The gustatory receptor homolog LITE-1 was identified in forward genetic screens for worms that failed to respond to blue light stimulation. When LITE-1 is expressed in body-wall muscles, it causes contraction in response to blue light suggesting that LITE-1 is both necessary and sufficient for blue light response. Here we show that in addition to light avoidance, LITE-1 is also required for worms' avoidance of high concentrations of diacetyl, an odorant that is attractive at low concentrations. Like blue light, diacetyl causes muscle contraction in transgenic worms engineered to express LITE-1 in body-wall muscles. These data are consistent with a direct chemoreceptor function for LITE-1 which would make it a multimodal sensor of aversive stimuli.

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