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Amyloid fibrils in Alzheimer's disease differently modulate sleep and cortical oscillations in mice depending on the type of amyloid

Sanagi, T.; Okumura, M.; Lin, Y.; Kanemura, S.; Moon, E.; Heo, Y.; Takahara, K.; Tsunematsu, T.; Lee, Y.-H.

2025-04-15 neuroscience
10.1101/2025.04.09.647928 bioRxiv
Show abstract

Alzheimers disease (AD) is characterized by aggregation and deposition of the amyloid-beta (A{beta}) protein in patient brains, with aging playing a crucial role through oxidative stress and neuroinflammation. Sleep disturbances are common in patients with AD, and contribute to their cognitive impairment. However, the association between the aggregation of specific A{beta} species in particular brain regions and its effect on sleep impairment remains unclear. Here, we investigated the effect of A{beta}1-40 (A{beta}40) and A{beta}1-42 (A{beta}42) amyloid fibrils on sleep/wakefulness and cortical oscillations in 3-month old wild-type mice. A{beta}42 aggregated faster and demonstrated distinct structural properties compared with A{beta}40. Bilateral injections into the dentate gyrus of the hippocampus showed that A{beta}42 amyloid fibrils significantly disrupted sleep and cortical activity as well as caused neuronal death, whereas A{beta}40 amyloid fibrils mainly affected cortical oscillations and caused minimal neuronal death. These findings shed light on AD-associated sleep disorders, which are differentially affected by the distinct properties of A{beta}40 and A{beta}42 aggregates.

Published in Biophysical Chemistry · training set

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