TUCL-Seq for miRNA single molecule sequencing
Fan, J.; Li, Z.; You, X.; Liu, L.; Jin, H.; Wu, P.; Han, Y.; Sun, L.; Xu, P.; Liu, Y.; Luo, J.; Yan, Q.
Show abstract
MicroRNAs (miRNAs) are pivotal regulatory molecules in gene expression, and their precise quantification is essential for elucidating their roles in diverse biological processes. This research paper introduces a pioneering workflow for the single molecule sequencing (SMS) of miRNA, integrating advanced miRNA sample preparation with SMS sequencing. Central to our approach is TUCL-Seq, which is an automated, on-chip miRNA library preparation method designed to streamline the sequencing process. This innovative approach incorporates the addition of sequencing adaptor to RNA samples in solution, and converts it to cDNA on a sequencing flow cell for sequencing. Coupled with single molecule sequencing instrument and base-call software, our workflow significantly reduces sample-to-result time, and decreases the need for manual interventions compared to traditional SMS and next-generation sequencing methods. We demonstrate the efficacy of TUCL-Seq by accurately quantifying synthetic miRNA samples with a 24-hour workflow, showcasing its potential for high-throughput miRNA profiling. Our findings highlight the promise of this integrated workflow for advancing miRNA research and applications in diagnostics and therapeutics.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Unlocking the Full Potential of Nanopore Sequencing: Tips, Tricks, and Advanced Data Analysis Techniques 95%
- Nanopore sequencing for N1-methylpseudouridine in RNA reveals sequence-dependent discrimination of the modified nucleotide triphosphate during transcription 94%
- Direct RNA sequencing (RNA004) allows for improved transcriptome assessment and near real-time tracking of methylation for medical applications 93%
Similar papers in this journal
Similar papers in this journal
- KaScape: A sequencing-based method for global characterization of protein-DNA binding affinity 94%
- Ready-to-use nanopore platform for the detection of any DNA/RNA oligo at attomole range using an Osmium tagged complementary probe. 93%
- Manatee: detection and quantification of small non-coding RNAs from next-generation sequencing data 93%
Similar papers in this journal
- Hairpin structure facilitates high-fidelity DNA amplification reactions in both qPCR and high-throughput sequencing 95%
- Well-Paired-Seq2: High-Throughput and High-Sensitivity Strategy for Characterizing Low RNA-Content Cell/Nucleus Transcriptomes 93%
- Nucleic acid quantification with amplicon yield in recombinase polymerase amplification 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.