Rapid slide-free histopathology via fluorescent layer-adaptive subcellular imaging: clinical-accessible platform for diverse tissues

2025-04-09 pathology Title + abstract only

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Intraoperative pathology remains constrained by ice crystal artifacts in frozen sections and the high cost of emerging slide-free optical methods. Here, we introduce FLASH-Path, a rapid slide-free technique enabling subcellular-resolution imaging of centimeter-scale tissues in 10 minutes. By replacing mechanical thin sectioning or optical thin-layer excitation with thin-layer ([≤]10 {micro}m) fluorescent labeling using commercially available probes, FLASH-Path achieves artifact-free visualiza...

Rapid slide-free histopathology via fluorescent layer-adaptive subcellular imaging: clinical-accessible platform for diverse tissues

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