Global characterization of Dictyostelium discoideum gene expression changes under hypoxic conditions
Hesnard, J.; Gas-Pascual, E.; van der Wel, H.; Cochet-Escartin, O.; Joly, S.; Rieu, J.-P.; West, C. M.; Anjard, C.
Show abstract
Aerobic eukaryotes utilize O2 to oxidize metabolites and generate ATP. The protist kingdom lacks the Hypoxia Inducible Factor-dependent transcriptional response network to accommodate low O2 though possesses a HIF prolyl hydroxylase that initially mediates the response. To address the scale and scope of hypoxic responses in protists, we characterized transcriptomic and proteomic changes when the social amoeba Dictyostelium is subjected to low (1%) O2 under nutritive conditions over 24 h followed by reoxygenation. Remarkably, 32% of the transcripts quantified were differentially expressed during hypoxia, with greatest changes associated with early (1 h) and late phases (24 h). Protein changes were modestly correlated with and generally lagged behind transcriptional changes. Correlated changes were observed for transcripts and proteins associated with various metabolic, anabolic, and catabolic pathways, as well as chromosome organization, cell cycling, vesicular trafficking, and signaling. Analysis of 4 marker genes showed extremely rapid responses that were graded over a range of O2 levels, with differential responses to inhibitors affecting protein synthesis and mitochondria. Overall, the amoebal response to a low but non-toxic O2-level resulted in massive remodeling of the transcriptome and proteome.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The mitochondrial Na+/Ca2+ exchanger NCLX is implied in the activation of hypoxia-inducible factors 93%
- Amino acid restriction sensitizes lung cancer cells toferroptosis via GCN2-dependent activation of the integratedstress response 92%
- Dynamic posttranslational modifications of cytoskeletal proteins unveil hot spots under nitroxidative stress 92%
Similar papers in this journal
- Multi-omic analysis of bat versus human fibroblasts reveals altered central metabolism 95%
- MYC overrides HIF to regulate proliferating primary cell metabolism in hypoxia 95%
- HIF-1α induces glycolytic reprogramming in tissue-resident alveolar macrophagesto promote survival during acute lung injury 94%
Similar papers in this journal
- Comparative analysis of N-terminal cysteine dioxygenation and prolyl-hydroxylation as oxygen sensing pathways in mammalian cells 93%
- H2S remodels mitochondrial ultrastructure and destabilizes respiratory supercomplexes 93%
- Pyruvate dehydrogenase kinase 1 controls triacylglycerol hydrolysis in cardiomyocytes 93%
Similar papers in this journal
- Identification of new interactors of eIF3f by endogenous proximity-dependent biotin labelling in human muscle cells. 92%
- Congruence between noise and plasticity in protein expression 91%
- MYCN-induced nucleolar stress drives an early senescence-like transcriptional program in hTERT-immortalized RPE cells 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.