AquIRE reveals multiple mechanisms of clinically induced RNA damage and the conservation and dynamics of glycoRNAs
Zhang, Z.; Kotopanova, Z. V.; Dang, K.; Kong, X.; Simms, N.; Yuen, T. W.; Sonmez, A.; Beadle, L. F.; Hilton, E.; Qureshi, T.; Coppola, M.; Holmes, C. D.; Kan, K. T.; Ashe, M.; Gallois, P.; Ashe, H.; Lafontaine, D. L.; Braun, M.; Saunders, M.; Sutton, P.; Thornton, D.; Knight, J. R.
Show abstract
RNA is subject to many modifications, from small chemical changes such as methylation through to conjugation of biomolecules such as glycans. As well as these endogenously written modifications, RNA is also exposed to damage induced by its environment. Certain clinical compounds are known to drive covalent modifications of RNA with a growing appreciation for how these affect function. To understand the regulation of these modifications we need a reliable, sensitive and rapid methodology for their quantification. Thus, we developed AquIRE and applied it to the analysis of drug-induced RNA damage, showing this to be widespread with intricate temporal dynamics. Using the same methodology we identify RNA:protein crosslinking and the rewriting of the epitranscriptome as a consequence of clinical RNA damage. We also demonstrate how liquid-liquid phase separation increases RNA damage and expand the horizons of the glycoRNA world across the kingdoms of life and into cell-free glycoRNA.
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