A new genome assembly of the pea cultivar Cameor provides resources for functional genomics and genetics
Kreplak, J.; Novak, P.; Robledillo, L. A.; AUBERT, G.; Imbert, B.; Kaur, P.; Gouil, Q.; Lopez-Roques, C.; Rodde, N.; BOUCHEZ, O.; Tayeh, N.; Macas, J.; Burstin, J.
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Significant improvements in sequencing technologies have allowed the development of more contiguous genome assemblies in many plant species. The pea genome is characterized by its richness in repeated elements and its long and complex centromeres. This makes its assembly challenging. In this paper, we present an improved version of the genome sequence of the French cultivar Cameor. This sequence was obtained by combining Nanopore and PacBio long-read sequencing, Hi-C contact maps and Bionano maps. The assembly of centromeres was refined using a combination of FISH and ultra-long Nanopore read analyses. Overall, Cameor_v2 genome assembly is a highly continuous pea genome assembly with small total gap size and a large contig N50. In this version, the orientation of chromosomes was revised according to internationally accepted karyotype rules. Gene annotation statistics indicated a high completeness of gene sequences, with most gene sequences with 3 and 5 UTR. This genome assembly with its associated data constitute a useful resource for pea genetics, comparative mapping and functional genomics.
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