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Social evolution and absence of olfactory function in larval honey bees

Peng, T.; Chen, Z.; Ahmed, A. C.; Feng, Q.; Yeo, S.; Han, H.-S.; Robinson, G. E.

2025-04-02 evolutionary biology
10.1101/2025.03.31.646396 bioRxiv
Show abstract

Social evolution made larval honey bees dependent on adult colony members for feeding. We therefore predicted they have diminished olfactory capabilities, and based on organismal resource conservation theory, also have downregulated olfactory receptor (OR) gene expression. Behavioral assays demonstrated that larvae cannot find food via olfaction and expressed very low levels of Orco, an essential gene for OR function. By contrast, larvae showed higher expression of Ir25a, an essential gene for multiple forms of sensory perception including gustation. These results suggest larvae rely on taste for feeding. In addition, considering that adult bees use OR-based olfaction extensively, they demonstrate strong developmental regulation of the OR system. Comparative transcriptomics of social and non-social insects further highlight the role of social evolution in shaping this sensory trait.

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