Bottlenecks and Resistance Evolution against an Antimicrobial 1 Peptide
Kavlak, O.; Linke, L.; Bittermann, E.; Franz, M.; Rolff, J.
Show abstract
Bottlenecks - a severe reduction in population size--are ubiquitous for bacteria in natural systems. For example, during the complete metamorphosis of insects, the gut microbiota is bottlenecked due to a massive secretion of immune effectors such as antimicrobial peptides (AMPs) into the gut. However, the effect of natural bottlenecks on resistance evolution of bacteria to AMPs is currently unknown. Here we measured the bottlenecking of the gut microbiota of G. mellonella, the greater wax moth. Based on these population estimates, we tested in vitro the influence of population size on the adaptation of E. coli against an AMP. We used wild-type E. coli and mutator strains with a 100-fold increase in mutation rates to partly disentangle population size from mutant supply. We found that large E. coli mutator strain populations evolved higher resistance than small populations. Population size, however, did not affect adaptation in wild-type strains. They were not able to evolve resistance under our experimental conditions. This shows that natural bottlenecks can benefit insect hosts by combating the evolution of AMP resistance to their resident gut microbiota, but this should also be applicable to infection in other organisms that use AMPs as an immune defense.
Matching journals
The top 12 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Evolution of bacterial persistence to antibiotics during a 50,000-generation experiment in an antibiotic-free environment 93%
- Idiosyncratic fitness costs of ampicillin-resistant mutants derived from a long-term experiment with Escherichia coli 91%
- Tamoxifen repurposing to combat infections by multidrug-resistant Gram-negative bacilli 90%
Similar papers in this journal
Similar papers in this journal
- Prophage regulation of Shewanella fidelis 3313 motility and biofilm formation: implications for gut colonization dynamics in Ciona robusta 93%
- Integration host factor regulates colonization factors in the bee gut symbiont Frischella perrara 93%
- Spatial and temporal coordination of Duox/TrpA1/Dh31 and IMD pathways is required for the efficient elimination of pathogenic bacteria in the intestine of Drosophila larvae 92%
Similar papers in this journal
- Negative interactions and virulence differences drive the dynamics in multispecies bacterial infections 93%
- The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes. 92%
- First experimental evidence for active farming in ambrosia beetles and strong heredity of garden microbiomes 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.