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Imputing missing minimum inhibitory concentration (MIC) values for Pseudomonas aeruginosa strains with a Denoising AutoEncoder

Denakpo, E.; Dias, N.; Pitout, J.; Naas, T.; Pillai, D. R.; Jay, F.; Iorga, B. I.

2025-03-26 microbiology
10.1101/2025.03.26.645597 bioRxiv
Show abstract

Pseudomonas aeruginosa is a problematic pathogen with complex antibiotic resistance patterns. In clinical practice, minimum inhibitory concentration (MIC) tests typically focus on a limited subset of antibiotics, hindering a comprehensive assessment of a strains resistance profile. Here, we introduce MICFiller, a Denoising AutoEncoder (DAE) model designed to impute missing MIC values for 14 antibiotics in Pseudomonas aeruginosa within a specific dilution range by leveraging known MIC measurements for other antibiotics in the same strain. We evaluated the performance of DAE against two other commonly used methods: Multiple Imputation by Chained Equations (MICE) and simple median imputation. The DAE achieved the highest balanced 1-tier accuracy for most antibiotics, with performance closely matching that of MICE. MICFiller is freely accessible through a user-friendly web interface at http://iorgalab.org:4567/micfiller, offering clinicians a more complete view of a strains antibiotic resistance profile.

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