Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines
Pavlinek, A.; Dutan-Polit, L.; Nagy, R.; APEX Consortia, ; Lancaster, M. A.; Vernon, A. C.; Srivastava, D. P.
Show abstract
Microelectrode arrays (MEAs) are increasingly used to profile the development of synchronised activity in neural organoids, yet no organoid study has investigated the consistency of electrophysiological development across cell lines. Here, we used dissociated neural organoids derived from four cell lines on MEAs to characterise functional synapse development using multiple parameters across time. The dissociated organoids had increasing functional connectivity and network activity over time across all cell lines and plasticity in response to synaptic-like stimulation. Like the organoids they were derived from, dissociated organoid cultures contained a diverse mixture of cell types. Variability in activity parameters was associated with differences in cell type composition and regional identity, which in turn were affected by donor cell line and batch effects. These results demonstrate that dissociated cerebral organoids can generate functional neurons, akin to primary neuronal cultures from brain tissue, providing a scalable model for studies of neurodevelopment and synaptic function.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Self-Organizing Neural Networks in Organoids Reveal Principles of Forebrain Circuit Assembly 95%
- A proper Excitatory/Inhibitory ratio is required to develop synchronized network activity in mouse cortical cultures 95%
- Hypersynchronous iPSC-derived SHANK2 neuronal networks are rescued by mGluR5 agonism 94%
Similar papers in this journal
- Variability vs Phenotype: multimodal analysis of Dravet Syndrome Brain Organoids powered by Deep Learning 94%
- Comparing the impact of sample multiplexing approaches for single-cell RNA-sequencing on downstream analysis using cerebellar organoids 93%
- Rich dynamics and functional organization on topographically designed neuronal networks in vitro 93%
Similar papers in this journal
- Lipopolysaccharide-induced neuroinflammation disrupts functional connectivity and community structure in primary cortical microtissues 94%
- Human Cerebral Spheroids Undergo Activity Dependent Changes In Cellular Composition And MicroRNA Expression 94%
- Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells 93%
Similar papers in this journal
- Synaptic and intrinsic membrane defects disrupt early neural network dynamics in Down syndrome 94%
- Versatile live-cell activity analysis platform for characterization of neuronal dynamics at single-cell and network level 94%
- Enhanced Production of Mesencephalic Dopaminergic Neurons from Lineage-Restricted Human Undifferentiated Stem Cells 93%
Similar papers in this journal
- Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmitters 94%
- Single-cell sequencing of individual retinal organoids reveals determinants of cell fate heterogeneity 92%
- Ultrastructural analysis of synapses after induction of spike-timing-dependent plasticity 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.