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Prior epigenetic status predicts future susceptibility to seizures in mice

Boros, B. D.; Gachechiladze, M. A.; Guo, J.; Galloway, D. A.; Mueller, S. M.; Shabsovich, M.; Yen, A.; Cammack, A. J.; Shen, T.; Mitra, R. D.; Dougherty, J. D.; Miller, T. M.

2025-03-23 genomics
10.1101/2025.03.20.644199 bioRxiv
Show abstract

Wide variation of responses to identical stimuli presented to genetically inbred mice suggests the hypothesis that stochastic epigenetic variation during neurodevelopment can mediate such phenotypic differences. However , this hypothesis is largely untested since capturing pre-existing molecular states requires non-destructive, longitudinal recording. Therefore, we tested the potential of Calling Cards (CC) to record transient neuronal enhancer activity during postnatal development, and thereby associate epigenetic variation with a subsequent phenotypic presentation - degree of seizure response to the pro-convulsant pentylenetetrazol. We show that recorded differences in epigenetics at 243 loci predict a severe vs. mild response, and that these are enriched near genes associated with human epilepsy. We also validated pharmacologically a seizure -modifying role for two novel genes, Htr1f and Let7c. This proof-of-principle supports using CC broadly to discover predisposition loci for other neuropsychiatric traits and behaviors. Finally, as, human disease is also influenced by non-inherited factors, similar epigenetic predispositions are possible in humans.

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