High-Quality Cell Capture Reveals Transcriptomic Changes After Single-Axon Injury of Mauthner Cells
Song, Z.; Zhu, L.; Shen, Y.; Yao, H.; Cai, Y.; Shi, L.; Chen, X.; Han, A.; Zhao, Z.; Qu, K.; Hu, B.
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In vivo single-cell capture methods based on micropipettes are essential for correlating morphological characteristics with transcriptomic profiling under physiological conditions. However, they often suffer from significant contamination of off-target cells. Consequently, we developed Hip-seq, which significantly reduces heavily contaminated cells, decreases contamination levels, improves cell reproducibility, and enhances data analysis accuracy compared to patch-seq. Using Hip-seq, we found that axon regeneration failure could be due to abnormal activation of translation and the circadian clock. Axon-regenerable central neurons reactivated axon development-related genes during regeneration. And one-to-one associated analysis helped identify pro-regenerative genes.
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