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NeoAtlas and NeoBert: A Database and A Predictive Model for Canonical and Noncanonical Tumor Neoantigens

Shi, M.; Yan, Q.; Zhao, W.; Teng, C.; Han, F.; Chen, H.; Li, Y.; Xu, L.; Yang, F.; Jin, G.; Bao, Y.; Zuo, C.; Li, J.

2025-03-17 systems biology
10.1101/2025.03.16.643518 bioRxiv
Show abstract

Neoantigens are classified into canonical and noncanonical types. Noncanonical neoantigens include those derived from noncoding regions, transposable elements (TE), and intron retention events, and they have recently gained considerable attention in cancer immunity. We curated 35,574 non-redundant neoantigen-HLA pairs from 14 immunopeptidomes studies, by analyzing unique features and differences across various sources of neoantigens. This knowledge enabled us to develop machine learning models for the prediction of different types of neoantigens. Our data and models are available at a public portal (https://ngdc.cncb.ac.cn/neoatlas) to facilitate broad access and future research. This resource offers advanced functionalities, including integration with epigenome browsers which allow easy navigation of epigenomic datasets to support and confirm the expression of neoantigens. We further demonstrate that combining our database with mass spectrometry analysis can identify noncanonical neoantigens. The resource we constructed holds significant value and promise for the development of neoantigen-based vaccines.

Published in Genomics, Proteomics & Bioinformatics (predicted rank #1) · training set

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