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Diverse crop rotations off-set yield-scaled nitrogen losses via denitrification

Saghai, A.; Smith, M. E.; Vico, G.; Banerjee, S.; Edlinger, A.; Garcia-Palacios, P.; Garland, G.; van der Heijden, M. G. A.; Herzog, C.; Maestre, F. T.; Pescador, D. S.; Philippot, L.; Rillig, M. C.; Romdhane, S.; Bommarco, R.; Hallin, S.

2025-03-17 ecology
10.1101/2025.03.15.643431 bioRxiv
Show abstract

Denitrification, a major source of gaseous nitrogen (N) emissions from agricultural soils, is influenced by management. Practices promoting belowground diversity are suggested to support sustainable agriculture, but their ability to modulate gaseous N-losses via denitrification remains inconclusive. To fill this knowledge gap, we sampled 106 cereal fields spanning a 3,000 km North-South gradient across Europe and compiled 56 associated climatic, soil, microbial and management variables. We found that increased denitrification was associated with higher proportion of time with crop cover over the last ten years. Denitrification rates were best predicted by microbial biomass and microbial functional guilds involved in N cycling, in particular denitrification. We also show that several diversification practices affect the variation in denitrification predictors, suggesting a trade-off between agricultural diversification and gaseous N-losses via denitrification. However, increased crop diversity in rotations improved yield-scaled denitrification, highlighting the potential of this practice to minimize N losses while contributing to sustainable food production.

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