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First-void urine and cervicovaginal brushes as sampling devices for vaginal microbiome studies: a proof-of-concept study

Pinedo-Bardales, M.; Van Caesbroeck, A.; Ahannach, S.; Bell, M.; Van Keer, S.; Teblick, L.; Erreygers, I.; Bakelants, S.; Van den Bosch, S.; Condori-Catachura, S.; Wittouck, S.; Vorsters, A.; Lebeer, S.

2025-03-15 microbiology
10.1101/2025.03.14.643287 bioRxiv
Show abstract

This proof-of-concept study compares first-void urine (FVU) and cervicovaginal brushes to vaginal swabs for microbiome profiling in ten women. FVU and brushes provided comparable results in most cases, though storage conditions influenced microbial composition, especially for FVU. Despite limitations in sample size and diversity in participants, findings support these methods potential for self-sampling in womens health research, emphasizing the need for optimized techniques to improve vaginal microbiome profiling.

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