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Machine learning inference of natural product chemistry across biosynthetic gene cluster types

Larralde, M.; Zeller, G.

2025-03-15 bioinformatics
10.1101/2025.03.13.642868 bioRxiv
Show abstract

With ever-increasing volumes of sequencing data for biosynthetic gene clusters (BGCs), computational methods for the prediction of resulting secondary metabolites are critically needed. Here, we present CHAMOIS, a machine learning tool inferring metabolite properties from protein domains in BGCs. Out of 539 relevant chemical properties from the ChemOnt ontology, CHAMOIS predicts 120 with an AUPRC > 0.5. Although entirely data-driven, CHAMOIS infers many protein-metabolite links that are consistent with the scientific literature and suggests interesting novel biosynthetic functions of uncharacterized proteins. Finally, to guide experimental BGC characterisation, CHAMOIS can pinpoint which BGC within a given genome produces a pre-specified metabolite.

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