O-GlcNAc modifications regulate lamin A tail processing
Augspurger, K. R.; Martin, E. A.; Maynard, J.; Welle, K.; Ghaemmaghami, S.; Burlingame, A.; Panning, B.; Buchwalter, A.
Show abstract
Lamin A processing is highly regulated, and necessary for proper assembly of the nuclear lamina facilitating its role in nuclear structure and chromatin organization. Pre-lamin A is first farnesylated, and then a short C-terminal peptide is cleaved to produce mature lamin A. O-GlcNAc Transferase (OGT), a glucose sensitive post-translational modification enzyme, is a potential regulator for lamin A processing. To explore the role of OGT in lamin A biogenesis, we examined the effects of OGT levels and OGT inhibition. Variation in OGT dose or inhibition of its activity did not alter endogenous lamin A abundance or distribution. To more directly test the regulatory effects of O-GlcNAcylation on lamin A, we adapted a tail cleavage assay. Mutation of an OGT binding motif and O-GlcNAc modification sites reduced tail cleavage efficiency, suggesting that O-GlcNAcylation promotes lamin A processing. Our findings add to the understanding of the regulation of lamin A cleavage and identify a potential link between glucose metabolism and lamina biogenesis.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Orchestration of SARS-CoV-2 Nsp4 and host-cell ESCRT proteins induces morphological changes of the endoplasmic reticulum 94%
- Protein-protein interactions with G3BPs drive stress granule condensation and gene expression changes under cellular stress 92%
- The Mitochondrial RNA Granule Modulates Manganese-Dependent Cell Toxicity 92%
Similar papers in this journal
- An in vivo 'turning model' reveals new RanBP9 interactions in lung macrophages. 94%
- An evolutionarily conserved role for separase in the regulation of nuclear lamins 93%
- ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions 92%
Similar papers in this journal
- Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development 94%
- FAM57B is a modulator of ceramide synthesis that regulates sphingolipid homeostasis and synaptic composition in the developing brain 93%
- STING Nuclear Partners Contribute to Innate Immune Signalling Responses 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.