Derivation of functional retinal endothelial cells from human pluripotent stem cells for therapeutics and modeling
Lin, Y.-Y.; Esswein, P.; Ramirez, L.; Warren, E.; Gerecht, S.
Show abstract
Retinal microvascular diseases involve a compromised inner blood-retina barrier (iBRB), which remains poorly understood. A renewable source of human iBRB endothelium is thus vital for advancing eye research and treatment development. Here, we differentiated human iPSCs into retinal endothelial cells (iRECs) via the Wnt/{beta}-catenin pathway, namely Norrin/Frizzled4 signaling. These iRECs show genetic, protein, and functional fidelity and unique retinal features. When injected into oxygen-induced retinopathy mice, iRECs integrated into the host vascular network and revascularized the ischemic eye, rescuing the tissue. Within microphysiological models, iRECs form perfusable microvascular networks that mimic the iBRBs morphology and phenotype in both health and diabetic retinopathy conditions while also interacting and organizing physiologically with iPSC-derived retinal pericytes. Our studies establish functional human iRECs and microphysiological iBRB models that facilitate mechanistic studies aimed at identifying therapeutic targets and promoting the revascularization of injured retinas, thereby supporting treatment advancement.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Circulating tumor cells shed shearosome extracellular vesicles in capillary bifurcations that activate endothelial and immune cells 93%
- Symmetry breaking of hPSCs in micropattern generates a polarized spinal cord-like organoid (pSCO) with dorsoventral organization 93%
- OPTRACE: Optical Imaging Guided Transplantation and Tracking of Cells in the Mouse Brain 93%
Similar papers in this journal
- Xenotransplantation of Human PSC-derived Microglia Creates a Chimeric Mouse Brain Model that Recapitulates Features of Adult Human Microglia 94%
- Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype 94%
- Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion, and drug screening 94%
Similar papers in this journal
- Specialized signaling centers direct cell fate and spatial organization in a limb organoid model 93%
- Xenogeneic Skin Transplantation Promotes Angiogenesis and Tissue Regeneration Through Vitamin D-Activated Trem2+ Macrophages 93%
- Injectable 3D microcultures enable intracerebral transplantation of mature neurons directly reprogrammed from patient fibroblasts 93%
Similar papers in this journal
- Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy 95%
- Glycocalyx-Mediated Vascular Dissemination of Circulating Tumor Cells 95%
- Ca2+ Oscillation in Vascular Smooth Muscle Cells Control Myogenic Spontaneous Vasomotion and Counteract Post-ischemic No-reflow 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.