Open EGGbox: an open-source 3D-printed embryonic Gallus gallus toolbox for electroporation and culture/live imaging of avian embryos ex ovo
Antoniou-Kourantonio, M.; Ramirez de Acuna, F.; Schettler, R. C. C.; Udar, S. K.; Hamzic Petite, A.; Sivasubramanian, R. V.; Munsterberg, A. E.; Grocott, T.
Show abstract
We present an open source, 3D-printed toolbox for avian embryology. The toolbox includes an electroporation chamber for transfecting functional molecular reagents into developing embryos, and a set of live-imaging chambers, which support avian embryo development while presenting them to a wide variety of microscope setups. We demonstrate both electroporation and imaging chambers by transfecting novel fluorescent reporter constructs for the TGF-beta signalling pathway and Pax7, Brachyury, Cdx2 and Sox2:Oct4 transcription factors into chick embryos and performing time-lapse imaging from stages HH3 - HH12 via both widefield and confocal fluorescence microscopy. Open-source code and ready-to-print STL files are freely available from a GitHub repository in line with FAIR (findability, accessibility, interoperability, and reusability) principles.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis 94%
- ECM-integrin signalling instructs cellular position-sensing to pattern the early mouse embryo 94%
- Cell polarity determinant Dlg1 facilitates epithelial invagination by promoting tissue-scale mechanical coordination 94%
Similar papers in this journal
- The Digital 3D-Atlas MAKER (DAMAKER): a dynamic and expandable digital 3D-tool for monitoring the temporal changes in tissue growth during hindbrain morphogenesis 95%
- Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach 94%
- A Versatile Oblique Plane Microscope for Large-Scale and High-Resolution Imaging of Subcellular Dynamics. 94%
Similar papers in this journal
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development 94%
- A versatile automated high-throughput drug screening platform for zebrafish embryos 94%
- Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function 92%
Similar papers in this journal
- Heterologous reporter expression in the planarian Schmidtea mediterranea through somatic mRNA transfection 94%
- Accelerated protein retention expansion microscopy using microwave radiation 94%
- Fast 3D Clear: A Fast, Aqueous, Reversible Three-Day Tissue Clearing Method for Adult and Embryonic Mouse Brain and Whole Body 93%
Similar papers in this journal
- LongAxis: a MATLAB-based program for 3D quantitative analysis of epithelial cell shape and orientation 95%
- CRISPR-Cas13d as a molecular tool to achieve targeted gene expression knockdown in chick embryos 95%
- An RNA interference approach for functional studies in the sea urchin and its use in analysis of Nodal signaling gradients 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.