A pipeline for screening condition-specific enzymes uncovers a function for the alcohol dehydrogenase Bdh2
Edilbi, D.; Valenti, R.; Dubreuil, B.; Asraf, Y.; Malitsky, S.; Itkin, M.; Schuldiner, M.
Show abstract
Cells possess intricate metabolic networks comprised of hundreds of enzymes. Despite extensive research, many of these enzymes remain uncharacterized. Identifying such enzymes is crucial for advancing our understanding of metabolism. However, multiple enzymes are not expressed in standard conditions, making them challenging to study. To overcome this challenge, we created a pipeline for characterizing the expression of condition-specific enzymes in yeast. We assembled a collection of 110 yeast strains, each containing an uncharacterized putative enzyme fused to a fluorophore under the regulation of their own promotor. By subjecting them to 43 diverse growth or stress environments, we identified the biologically relevant conditions for the expression of 19 proteins. We focused on one such putative alcohol dehydrogenase, Bdh2, and functionally characterized it. More broadly, our discovery pipeline lays the foundation for uncovering new condition-specific enzymes. This has implications in cell biology and biotechnology and should expand our understanding of metabolism.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Ribo-seq guided design of enhanced protein secretion in Komagataella phaffii 97%
- Evolution guided tolerance engineering of Pseudomonas putida KT2440 for production of the sustainable aviation fuel precursor isoprenol 96%
- Yeast-based heterologous production of the Colletochlorin family of fungal secondary metabolites 96%
Similar papers in this journal
Similar papers in this journal
- How to characterize a strain? The neglected influence of clonal heterogeneity on the phenotypes of industrial Saccharomyces 94%
- A key role of the EMC complex for mitochondrial respiration and quiescence in fission yeasts 94%
- MKT1 alleles regulate stress responses through post-transcriptional modulation of Puf3 targets in budding yeast 93%
Similar papers in this journal
- Calcium starvation leads to strain-specific gene regulation of lipid and carotenoid production in Mucor Circinelloides 95%
- One-carbon metabolic enzymes are regulated during cell division and make distinct contributions to the metabolome and cell cycle progression in Saccharomyces cerevisiae 94%
- Systematic Profiling of Ale Yeast Protein Dynamics across Fermentation and Repitching 93%
Similar papers in this journal
- The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking 94%
- Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa 92%
- Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa. 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.