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Seek-a-Seq: Optical detection of target nucleic acids with attomolar sensitivity

Das, A. K.; Saj, A.; Gan, H. H.; Gunsalus, K. C.; Shubeita, G. T.

2025-02-21 biophysics
10.1101/2025.02.18.638747 bioRxiv
Show abstract

We introduce Seek-a-Seq, an enzyme-free, PCR amplification-free, optical method to detect microbial nucleic acids based on fluorescent readout of hybridization between target sequences and complementary probes immobilized on the surface of customized sample chambers. We demonstrate the ability to detect ultralow concentration (~ 1 attomolar) of short target DNA and synthetic SARS-CoV-2 RNA with ease and specificity. Seek-a-Seq can enormously reduce costs, accelerate diagnostic workflows, and outperform existing enzyme-dependent methods.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.