DDX5 and DDX17 RNA helicases regulate hepatitis B virus RNA splicing
Giraud, G.; Grand, X.; Huchon, P.; Roda, M.; Diederichs, A.; Chapus, F.; De Nicola, F.; Parent, R.; Rivoire, M.; Bourgeois, C. F.; Zoulim, F.; Testoni, B.
Show abstract
Chronic HBV infection remains a major health burden worldwide and is the main driver of severe liver diseases. Liver pathogenesis is associated with the increased proportion of HBV spliced variants that encode viral proteins involved in liver disease progression. However, how HBV RNA splicing is regulated is poorly understood. Here, we focused on DDX5 and DDX17 RNA helicases, known to regulate HBV RNA metabolism and alternative splicing of host genes. By performing 5RACE-PCR combined with single molecule sequencing, we demonstrated that silencing both proteins increased the usage of a specific splicing donor site and the expression of the derived HBV spliced variants. Polysome fractionation highlighted the ability of these RNA species to encode new viral proteins potentially contributing to liver pathogenesis. Overall, our data established DDX5 and DDX17 helicases as master regulators of HBV RNA metabolism, by fine-tuning viral splicing, which is linked to HBV-induced liver pathogenesis and disease progression.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A conserved long-range RNA interaction in SARS-CoV-2 recruits ADAR1 to enhance virus proliferation 95%
- N6-methyladenosine modification is not a general trait of viral RNA genomes 95%
- Saudi Arabian SARS-CoV-2 genomes implicate a mutant Nucleocapsid protein in modulating host interactions and increased viral load in COVID-19 patients 94%
Similar papers in this journal
- Nanopore ReCappable Sequencing maps SARS-CoV-2 5' capping sites and provides new insights into the structure of sgRNAs 95%
- Investigating molecular mechanisms of 2A-stimulated ribosomal pausing and frameshifting in Theilovirus 94%
- The differential effect of SARS-COV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage and virus evolution 94%
Similar papers in this journal
Similar papers in this journal
- Coordination of transcriptional and translational regulations in human cells infected by <em>Listeria monocytogenes</em> 93%
- Subcellular relocalization and nuclear redistribution of the RNA methyltransferases TRMT1 and TRMT1L upon neuronal activation 91%
- Biochemistry-informed design selects potent siRNAs against SARS-CoV-2 91%
Similar papers in this journal
- A genome-wide CRISPR screen identifies ZCCHC14 as a host factor required for hepatitis B surface antigen production 96%
- The N-terminal and central domains of CoV-2 nsp1 play key functional roles in suppression of cellular gene expression and preservation of viral gene expression 93%
- Genome-wide CRISPR screens identify noncanonical translation factor eIF2A as an enhancer of SARS-CoV-2 programmed -1 ribosomal frameshifting 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.