Multiple Xpa In vivo crystallization routes in HEK 293 human cells
Leymarie, O.; Skobelkina, A.; montaville, p.; Brewee, C.; Isabet, T.; Chauvet, H.; Pereiro, E.; Chavas, L. M.; Jamme, F.
Show abstract
Phase changes of macromolecules in living cells gained recently a major interest in cell biology as liquid liquid phase separation or liquid solid phase transition phenomenon being observed in increasing number of biological or pathological processes. Comprehensive characterization of these phases remains challenging and requires new methodological approaches for their complete description across spatial and temporal scales. We propose a combination of imaging methods applied to a model system and report the in vivo crystallization pathways of a macromolecule from its solution to crystalline states at the cell population level down to the meso scale. Combining various live fluorescence based techniques and a high resolution cryo imaging technique within unaltered cryopreserved cells, we could described the unexpectedly wide landscape of the in vivo crystalline states of the fluorescent coral derived protein xpa, gain information of crystal growth dynamics in cellulo, and provide hypothesis of the crystal nucleation requirements of this stochastic process.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A native chemical chaperone in the human eye lens 92%
- Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts 92%
- Protein-lipid interaction at low pH induces oligomerisation of the MakA cytotoxin from Vibrio cholerae 92%
Similar papers in this journal
Similar papers in this journal
- Ice thickness control and measurement in the VitroJet for time-efficient single particle structure determination 93%
- Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy 92%
- Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.