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Stable Actin As Synaptic Tag

Thomas, M.; Bogaciu, C.-A.; Rizzoli, S.; Fauth, M.

2025-02-10 neuroscience
10.1101/2025.02.10.637360 bioRxiv
Show abstract

According to the tagging and capture hypothesis, long-lasting long-term potentiation (LTP) requires protein synthesis and a synaptic tag, which is a synapse specific memory of the synapse with a so far unclear molecular or biophysical identity. Here we use an interdisciplinary approach to explore the hypothesis that interaction between the dynamics of actin and the spine geometry can provide such a memory. Using a mathematical model, we demonstrate that this implementation of the tag requires an increase in the stable, cross-linked pool of actin filaments, and is not possible without this stable pool. Using FRAP experiments, we show that such an increase in stable actin can indeed be observed after cLTP in vitro. Thus, the interaction between actin dynamics and spine geometry could indeed serve as a synaptic tag for LTP.

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