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MAIT cells protect in severe pneumococcal pneumonia by regulating neutrophil/macrophage antimicrobial activities

Barsac, E.; Gonzalez, L.; Ilango, G.; Hankard, A.; Jouan, Y.; Lemoine, R.; Minhas, V.; Lenain, M.; Benard, M.; Fernandez, M.; Gaudio, A.; Si-Tahar, M.; Guillon, A.; Mallevaey, T.; Ganskow, C. S. G.; Klahn, P.; Veening, J.-W.; Salou, M.; Lantz, O.; Baranek, T.; Paget, C.

2025-02-08 immunology
10.1101/2025.02.07.637027 bioRxiv
Show abstract

Mucosal-Associated Invariant T (MAIT) cells populate the lung tissue where they contribute to defense against respiratory infections. While MAIT cells have been implicated in host resistance to infections caused by Gram-negative bacteria, their contribution in immunity against Gram-positive bacteria-driven pneumonia is still enigmatic. Here, we demonstrate that both mouse and human MAIT cells are activated during severe infection caused by Streptococcus pneumoniae, the major cause of community-acquired bacterial pneumonia. Upon infection, lung MAIT cells undergo a transcriptional reprogramming associated with acquisition of potent antimicrobial properties. MAIT cell-deficient mice are more susceptible to pneumococcal pneumonia, including higher mortality, uncontrolled bacterial growth and dissemination, and impaired neutrophil and interstitial macrophage activity. Moreover, prophylactic stimulation of MAIT cells using cognate antigen protects from pneumococcus-induced lethal pneumonia. These findings demonstrate that MAIT cells are key cellular actors during Gram-positive bacterial infections.

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