A Multiplex qPCR to Differentiate Monkeypox Virus Clades
Williams, C. T.; Romero Ramirez, A. I.; Semiu, A. A.; Ifabumuyi, S. O.; Gould, S. I.; Wooding, D.; Allen, C.; Somasundaran, A.; Mkpuma, N. N.; Gado, D.; Adole, J. A.; Amoo, A. E.; Adeyemi, A. A.; de Jarcy, L. B.; Goffinet, C.; Dunning, J.; Semple, M. G.; ISARIC CCP UK investigators, ; Bahizire, E.; Akinpelu, A.; Fletcher, T.; Cubas Atienzar, A. I.; Leggio, C.; Adedeji, A.; Omoare, A. A.; Edwards, T. I.
Show abstract
We designed a multiplex qPCR to differentiate monkeypox virus clades. In evaluations using clinical samples collected in the UK and Nigeria, the assay had an overall sensitivity of 78% (95% CI: 67.67% to 86.14%) and specificity of 94% (95% CI: 80.84% to 99.30%); for samples under Ct35 sensitivity was 98% (95% CI: 91.72% to 99.96%) and specificity was 94% (95% CI: 80.84% to 99.30%).
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