Bacteriophage purification using CIMmultus monolithic OH-column chromatography for therapeutic purposes
Echterhof, A.; Dhamaraj, T.; Blankenberg, P.; Hajfathalian, M.; Blankenberg, F.; Sacher, J.; Bollyky, P.
Show abstract
Bacteriophages are increasingly explored as antibacterial agents for therapeutic applications and pathogen biocontrol. Rigorous quality control and removal of microbial growth byproducts is essential for safety and compliance with regulatory standards for the use of phages; for phages isolated from Gram-negative bacterial lysates, this involves removal of endotoxin (lipopolysaccharide/LPS) and contaminating host proteins. Here, we describe the development of CIMmultus OH-Monolithic chromatography as a single-step purification process for five tailed anti-pseudomonal phages, including myoviral and siphoviral morphologies, as well as so-called "jumbo" phages, yielding therapeutically compliant samples suitable for human IV administration. Using preferential exclusion chromatography with a potassium phosphate gradient, we achieved near-complete removal of endotoxin (99.96-100.00% depletion) and protein impurities (95.8-99.7% depletion). We found that phage recovery post-purification was inversely associated with tail length and hydrodynamic diameter, with shorter-tailed and smaller phages demonstrating greater recovery. We conclude that single-step CIMmultus OH-monolithic chromatography is scalable, reproducible, and free from hazardous chemicals, supporting its integration into industrial phage purification processes.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Removal of Clinically Relevant SARS-CoV-2 Variants by An Affinity Resin Containing Galanthus nivalis Agglutinin 94%
- High-yield Production of Recombinant Platelet Factor 4 Protein by Harnessing and Honing the Gram-negative Bacterial Secretory Apparatus 93%
- Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced in Hansenula polymorpha 93%
Similar papers in this journal
- Low-Cost In-House Re-formulated Brain Heart Infusion Medium for Effective Planktonic Growth and Early Detection of Bloodstream Bacterial Pathogens 93%
- Phage-Derived Depolymerase as an Antibiotic Adjuvant Against Multidrug-Resistant Acinetobacter Baumannii 93%
- Cas12a-based on-site and rapid nucleic acid detection of African swine fever 92%
Similar papers in this journal
- Genome sequencing of the bacteriophage CL31 and interaction with the host strain Corynebacterium glutamicum ATCC 13032 92%
- High throughput Next-Generation Sequencing Respiratory Viral Panel: A Diagnostic and Epidemiologic Tool for SARS-CoV-2 and Other Viruses. 92%
- Combining short and long read sequencing technologies to identify SARS-CoV-2 variants in wastewater 92%
Similar papers in this journal
- A Rapid SARS-CoV-2 Variant Detection by Molecular-Clamping Based RT-qPCR 93%
- RT-LAMP-CRISPR-Cas13a technology as a promising diagnostic tool for the SARS-CoV-2 virus 92%
- Generation of false positive SARS-CoV-2 antigen results with testing conditions outside manufacturer recommendations: A scientific approach to pandemic misinformation 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.