Platelet endocytosis and α-granule cargo packaging are essential for normal skin wound healing

The high prevalence of chronic wounds, i.e., 2.5-3% of the US population, causes a large social and financial burden. Physiological wound healing is a multi-step process that involves different cell types and growth factors. Platelet-rich plasma or platelet-derived factors have been used to accelerate wound repair, but their use has been controversial with mixed results. Thus, a detailed functional understanding of platelet functions in wound healing beyond hemostasis is needed. This study investigated the importance of platelet -granule cargo packaging and endocytosis in a dorsal full-thickness excisional skin wound model using mice with defects in -granule cargo packaging (Nbeal2-/- mice) and endocytosis (platelet-specific Arf6-/- and VAMP2/3{Delta} mice). We found that proper kinetic and morphological healing of dorsal skin wounds in mice requires both de novo as well as endocytosed platelet -granule cargo. Histological and morphometric analyses of cross-sectional wound sections illustrated that mice with defects in -granule cargo packaging or platelet endocytosis had delayed (epi)dermal regeneration in both earlier and advanced healing. This was reflected by reductions in wound collagen and muscle/keratin content, delayed scab formation and/or resolution, re-epithelialization, and cell migration and proliferation. Molecular profiling analysis of wound extracts showed that the impact of platelet function extends beyond hemostasis to the inflammation, proliferation, and tissue remodeling phases via altered expression of several bioactive molecules, including IL-1{beta}, VEGF, MMP-9, and TIMP-1. These findings provide a basis for advances in clinical wound care through a better understanding of key mechanistic processes and cellular interactions in (patho)physiological wound healing. Key pointsO_LIDe novo and endocytosed platelet -granule cargo support physiological skin wound healing C_LIO_LIPlatelet function in wound healing extends to the inflammation, proliferation, and tissue remodeling phases C_LI