URAdime - a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons.
Limberis, J. D.; Metcalfe, J. Z.
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Targeted sequencing of multiplex PCR amplicons is commonplace in research laboratories and clinical diagnostics. There are numerous tools for the a priori optimization of primers and reactions, but no tools to detect specific problematic primers post hoc. We developed URAdime, a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons. We show that it successfully detects these unwanted amplicons and accurately attributes their generation to specific primers.
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