N-terminal modification of Histone H3 inhibits H3K27M-mediated loss of H3K27 trimethylation
Poux, N.; Zhang, D. F.; Kebede, A. F.; Gainey, I.; Famous, O. E.; Beroukhim, R.; Bandopadhayay, P.
Show abstract
The use of short epitope tags is widespread in the study of histone H3 biology as they allow for the antibody-mediated detection and pulldown of particular histone post-translational modifications (PTM) or exogenous histone transgenes. However, H3 is particularly sensitive to sequence modification and the addition of epitope tags may interfere with the native function of H3. Here, we use the known relationship between lysine-to-methionine K27M mutations and the loss of trimethylation at the H3 K27 residue to test whether the addition of epitope tags to the N or C terminus of H3 affects the levels of histone H3 PTMs. We find that all tested N-terminal tags abrogate the H3K27M-mediated loss of H3K27 trimethylation. These results suggest that the addition of epitope tags to the N-terminus of H3 should be performed with caution, and these findings may be of particular interest for the study of H3-driven cancers, like diffuse midline gliomas.
Matching journals
The top 12 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Generation of isogenic models of Angelman syndrome and Prader-Willi syndrome in CRISPR/Cas9-engineered human embryonic stem cells 93%
- Immortalization and Characterization of Schwann Cell Lines Derived from NF1 Associated Cutaneous Neurofibromas 93%
- Oncogenic mutation or overexpression of oncogenic KRAS or BRAF is not sufficient to confer oncogene addiction. 92%
Similar papers in this journal
- Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing is associated with therapeutic response to splicing inhibitor 95%
- The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program 94%
- Regulation of nerve growth and patterning by cell surface protein disulphide isomerase 94%
Similar papers in this journal
- Inhibition of HDAC7 reprograms the histone H3.3 landscape to induce heterochromatin spreading and DNA replication defects in cancer cells. 94%
- Modulation of prion protein expression through cryptic splice site manipulation 93%
- The Cancer Testis Antigen Testis Specific Serine Kinase 6 (TSSK6) is abnormally expressed in colorectal cancer and promotes oncogenic behaviors 93%
Similar papers in this journal
- Genome-Wide Screens Identify Core Regulators of Cell Surface Prion Protein Expression 94%
- Tagging allows faithful tracing of expression and enhances biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. 94%
- Transcriptomic analysis of melanoma cells reveals an association of α-synuclein with regulation of the inflammatory response. 93%
Similar papers in this journal
- Abundance and localization of human UBE3A protein isoforms 94%
- MSH2 knock-down shows CTG repeat stability and concomitant upstream demethylation at the DMPK locus in myotonic dystrophy type 1 human embryonic stem cells 94%
- Spinocerebellar Ataxia Type 1 protein Ataxin-1 is signalled to DNA damage by Ataxia Telangiectasia Mutated kinase 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.