In vitro antioxidant and anti-inflammatory activities, total phenolic and flavonoid contents of Anadenanthera colubrina from Northern Peru
Samame-Caramutti, E. A.; Salcedo Valdez, L.; SUAREZ-CUNZA, S.
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The antioxidant and anti-inflammatory activities of the hydroethanolic extract from bark of Anadenanthera colubrina (ACE), as well as its total phenolic (TPC) and flavonoid (TFC) contents, were studied in vitro. The ACE showed a high antioxidant activity both in the DPPH[bullet] radical scavenging (747.6mg Trolox equivalents/g dry extract) and in the FRAP (435.9mg FeSO4 equivalents/g dry extract) chemical assays. The extract (50 to 1000g/mL) inhibited the lipid peroxidation (formation of TBARS), hemolysis induced by H2O2, and egg albumin denaturation. No activity was observed in the hypotonicity-induced hemolysis assay. The TPC and TFC were 642.7mg GAE/g and 416.2mg CE/g dry extract, respectively. To the best of our knowledge, these activities were reported for the first time for A. colubrina from northern Peru, and suggest that the ACE has notable antioxidant and anti-inflammatory properties, and that the high TPC and TFC could be responsible, at least partially, for these activities.
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