Assessment of SARS-CoV2 genome sequence recovery from four lateral flow device products available in the UK.
Lewandowski, K.; Stokes, M.; Alexandridou, A.; Fenwick, A.; White, M. L.; Crook, J.; Rogers-Broadway, K.-R.; Ryan, C.; Williamson, D.; Vipond, R.; Pullan, S. T.
Show abstract
Lateral flow tests have played a key role in the response to the COVID-19 pandemic and are likely to be a major component of diagnostic strategies to combat future outbreaks of infectious disease. One challenge posed by widescale use of lateral flow tests in the community is the loss of sequence information to track virus evolution and epidemiology. Beyond their primary diagnostic function, it has been demonstrated that recovery of viral RNA for genome sequencing purposes is possible, from positive lateral flow devices (LFDs). To assess the robustness and broader applicability of this process, we assessed SARS-CoV-2 RNA recovery and sequencing from the four major LFDs in use in the UK. Testing both cultured virus and residual clinical nasal swab samples demonstrated that sequencing from LFD eluates is possible, at clinically relevant titres, within a reasonable processing time frame post-use, and gave sequences concordant with routine methods, but results varied across the four devices used. This highlights the requirement for refinement of existing LFDs or of second-generation LFD design, where sequencing is an intended output from positive LFDs.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Performance of the Cue COVID-19 Molecular Test for Point of Care: Insights from a multi-site clinic service model 95%
- Analytical comparison of over-the-counter multiplex tests for influenza A, influenza B and SARS-CoV-2 94%
- A Reagent and Virus Benchmarking Panel for a Uniform Analytical Performance Assessment of N Antigen–Based Diagnostic Tests for COVID-19 93%
Similar papers in this journal
- Is the glass half full? Extraction-free RT-LAMP to detect SARS-CoV-2 is less sensitive but highly specific compared to standard RT-PCR in 101 samples 93%
- Impaired performance of SARS-CoV-2 antigen-detecting rapid tests at elevated temperatures 93%
- Handling and accuracy of four rapid antigen tests for the diagnosis of SARS-CoV-2 compared to RT-qPCR 93%
Similar papers in this journal
- SARS-CoV-2 Antibody Lateral Flow Assay for antibody prevalence studies following vaccine roll out: a Diagnostic Accuracy Study 91%
- ARTIC RSV amplicon sequencing reveals global RSV genotype dynamics. 90%
- Sensitivity of RT-PCR testing of upper respiratory tract samples for SARS-CoV-2 in hospitalised patients: a retrospective cohort study. 89%
Similar papers in this journal
- Comparative sensitivity evaluation for 122 CE-marked SARS-CoV-2 antigen rapid tests 94%
- Establishment of an evaluation panel for the decentralized technical evaluation of the sensitivity of 31 rapid detection tests for SARS-CoV-2 diagnostics 93%
- An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage 91%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.