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Distinct memory CD4 subset tropism of two CCR5-tropic HIV-1 in a rapid progressor

Marichannegowda, M.; Farah, Y.; Bose, M.; Sanders-Buell, E.; King, D.; Francisco, L.; Eller, L. A.; Tovanabutra, S.; Michael, N. L.; Robb, M. L.; Song, H.

2025-01-23 microbiology
10.1101/2025.01.21.634053 bioRxiv
Show abstract

CCR5 tropic HIV-1 mainly infect memory CD4+ T cells. Memory CD4+ T cells are heterogenous which comprise central memory (CM), transitional memory (TM) and effector memory (EM) subsets based on cell differentiation stages. Previous studies demonstrate that low viral burden in the CM subset correlates with long-term non-progressive HIV-1 infection (1, 2) and is a hallmark of non-pathogenetic SIV infection (3, 4). These findings indicate the importance of CD4 subset tropism of HIV/SIV in determining disease progression. However, an important, yet unanswered question is whether CCR5 HIV-1 variants have different memory CD4 subset preferences. Here, we demonstrate clear compartmentalization of two CCR5 HIV-1 in different memory CD4 subsets in vivo.

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