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Propofol Treatment Alters the Metabolic State of MDA-MB-231 Breast Cancer Cells

Tasmi, T. A.; Holla, S.; Walsh, A.

2025-01-19 bioengineering
10.1101/2025.01.15.633222 bioRxiv
Show abstract

The mechanisms of many anesthetic drugs, such as propofol, are unknown. This study investigates the impact of propofol, a widely used intravenous anesthetic drug, on the metabolic behavior of the human triple-negative breast cancer cell line, MDA-MB 231. Utilizing fluorescence lifetime imaging microscopy (FLIM), we assessed the effect of propofol on cellular metabolism through imaging of the fluorescence lifetimes of NADH and FAD, coenzymes of metabolism reactions. Exposure to propofol-induced significant morphological and metabolic changes of the cells, including substantial cellular shrinkage, which may reveal a metabolic component to propofols mechanism.

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