Joint single-cell measurements of surface proteins, intracellular proteins and gene expression with icCITE-seq
Chen, K. Y.; Takeshima, Y.; Zorzetto-Fernandes, A. L.; Makino, K.; Kibayashi, T.; Hata, M.; Ichiyama, K.; Croteau, J.; Taylor, K.; Sakaguchi, S.
Show abstract
The development of single-cell RNA-sequencing assays has transformed our understanding of cellular and tissue heterogeneity, yielding significant insights into disease biology and its underlying mechanisms. In this work, we describe icCITE-seq (intracellular cellular indexing of transcriptomes and epitopes), a scalable method that simultaneously measures surface and intracellular protein levels alongside gene expression across thousands of cells. We validate the specificity of intracellular staining and demonstrate the utility of this multi-omic approach in interrogating phenotypic cellular states through targeted genetic perturbations in primary human T cells. icCITE-seq enables systematic profiling of gene expression, coupled with cytoplasmic, nuclear and PTM epitopes, providing an integrated approach towards understanding cellular identity, complexity and disease regulatory mechanisms.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer 99%
- CTCF and transcription influence chromatin structure re-configuration after mitosis 99%
- Allele-resolved single-cell multi-omics uncovers the dynamics and transcriptional kinetics of X-chromosome upregulation 98%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.