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Molecular dynamics guided engineering of Aequorea victoria Green Fluorescent Protein chromophore interactions generates a brighter variant with improved photobleaching resistance

Ahmed, R.; Jamieson, D.; Vitsupakorn, D.; Zitti, A.; Pawson, K.; Castell, O.; Watson, P.; Jones, D.

2025-01-09 biochemistry
10.1101/2025.01.08.631926 bioRxiv
Show abstract

Fluorescent proteins (FPs) are a crucial tool for cell imaging, but with developments in fluorescence microscopy and researcher requirements there is still a need to develop brighter versions that remain fluorescent for longer. Using short time-scale molecular dynamics-based modelling to predict changes in local chromophore interaction networks and solvation, we constructed an Aequorea victoria GFP (avGFP) variant called YuzuFP that is 1.5 times brighter than the starting superfolding variant (sfGFP) with a near 3-fold increased resistance to photobleaching in situ. YuzuFP contained a single mutation that replaces the chromophore interacting residue H148 with a serine. Longer time scale molecular dynamics revealed the likely mechanism of action is S148 makes more persistent polar interactions with the chromophore phenol group and increases the residency time of an important water molecule. As demonstrated by live cell imaging, YuzuFP not only offers a timely upgrade as a useful green-yellow avGFP for cell imaging applications over longer timescales, but it also provides a basic scaffold for future avGFP engineering efforts.

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