Cell cycle-regulated transcriptional pausing of Drosophila replication-dependent histone genes
Kemp, J. P.; Geisler, M. S.; Hoover, M.; Cho, C.-Y.; O'Farrell, P. H.; Marzluff, W. F.; Duronio, R. J.
Show abstract
Coordinated expression of replication-dependent (RD) histones genes occurs within the Histone Locus Body (HLB) during S phase, but the molecular steps in transcription that are cell cycle regulated are unknown. We report that Drosophila RNA Pol II promotes HLB formation and is enriched in the HLB outside of S phase, including G1-arrested cells that do not transcribe RD histone genes. In contrast, the transcription elongation factor Spt6 is enriched in HLBs only during S phase. Proliferating cells in the wing and eye primordium express full-length histone mRNAs during S phase but express only short nascent transcripts in cells in G1 or G2 consistent with these transcripts being paused and then terminated. Full-length transcripts are produced when Cyclin E/Cdk2 is activated as cells enter S phase. Thus, activation of transcription elongation by Cyclin E/Cdk2 and not recruitment of RNA pol II to the HLB is the critical step that links histone gene expression to cell cycle progression in Drosophila.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Levels of Notch-regulated transcription are modulated by tissue movements at gastrulation 96%
- Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activity 96%
- GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo 96%
Similar papers in this journal
- Orb-dependent polyadenylation contributes to PLP expression and centrosome scaffold assembly 97%
- Dynamics of hunchback translation in real time and at single mRNA resolution in the Drosophila embryo 96%
- Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation 96%
Similar papers in this journal
- A region of Drosophila SLBP distinct from the histone pre-mRNA binding and processing domains is essential for deposition of histone mRNA in the oocyte 97%
- Wash and the WASH Regulatory Complex function in Nuclear Envelope budding 97%
- The dual Ras Association (RA) Domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis 96%
Similar papers in this journal
- Defining the roles of the Integrator, NEXT, and nuclear exosome complexes in Drosophila oogenesis 97%
- Bicoid-nucleosome competition sets a concentration threshold for transcription constrained by genome replication 96%
- Mandatory coupling of zygotic transcription to DNA replication in early Drosophila embryos 96%
Similar papers in this journal
- The coordinated localization of mRNA to centrosomes facilitates error-free mitosis 96%
- Centriole growth is not limited by a finite pool of components, but is limited by the Cdk1/Cyclin-dependent phosphorylation of Ana2/STIL 96%
- The Ribb-osome: Ribbon boosts ribosomal protein gene expression to coordinate organ form and function 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.