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Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology

Passmore, J.; Rates, A.; Schroeder, J.; Van Laarhoven, M.; Hellebrekers, V.; Van Hoef, H.; Geurts, A.; Van Straaten, W.; Nijenhuis, W.; Berger, F.; Smith, C.; Smal, I.; Kapitein, L.

2024-12-17 cell biology
10.1101/2024.12.12.628240 bioRxiv
Show abstract

Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present outcome-driven microscopy, which combines smart microscopy with optogenetics to achieve subcellular spatiotemporal control of biology to predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, and demonstrate unprecedented spatiotemporal control over cellular behaviour.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.