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Sexual dimorphism in molecular profiles of resting human skeletal muscle and the response to acute exercise and endurance training

Dreher, S. I.; Goj, T.; von Toerne, C.; Hoene, M.; Irmler, M.; Ouni, M.; Jaehnert, M.; Beckers, J.; Hrabe de Angelis, M.; Peter, A.; Moller, A.; Birkenfeld, A. L.; Schuermann, A.; Hauck, S. M.; Weigert, C.

2024-12-14 biochemistry
10.1101/2024.12.12.627717 bioRxiv
Show abstract

Biological sex has a strong impact on skeletal muscle metabolism and performance. By a comprehensive investigation of epigenetic, transcriptomic and proteomic differences between female and male skeletal muscle of untrained subjects we provide a molecular basis for the sexual dimorphism of glucose and lipid metabolism. The sex-specific multi-OMICs profiles indicate higher degree of glucose turnover and higher abundance of fast-twitch fibers in males and high degree of lipid handling in females. Eight-week endurance training equalized initial differences toward an endurance-trained proteomic profile in both sexes. The untrained muscle of females was more resistant to an acute exercise challenge since stress-responsive transcripts were predominantly upregulated in males. In myotubes from the same donors, transcriptomic differences were hardly conserved, but could be partially restored by treatment with sex hormones. In conclusion, after only 8 weeks training mitigates deeply rooted sex-specific molecular profiles in skeletal muscle toward a common metabolically beneficial response.

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