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KlebPhaCol: A community-driven resource for Klebsiella research identified a novel gut phage order associated with the human gut

Rothschild-Rodriguez, D.; Lambon, K. S.; Latka, A.; Costa, A. R.; Mantzouratou, A.; King, C.; Boeckaerts, D.; Sheridan, E.; Merrick, F.; Drobniewski, F.; De Angelis, I.; Saeed, K.; Sutton, J. M.; Wand, M. E.; Andrew, M.; Hedges, M.; Brouns, S. J. J.; Haas, P.-J.; Kushwaha, S. K.; Lawson, S. T.; Fordham, S. M. E.; Lee, Y.-J.; Wu, Y.; Briers, Y.; Weigele, P. R.; Nobrega, F. L.

2024-12-02 microbiology
10.1101/2024.12.02.626457 bioRxiv
Show abstract

The growing threat of multidrug-resistant Klebsiella pneumoniae, coupled with its role in gut colonisation, has intensified the search for new treatments, including bacteriophage therapy. Despite increasing documentation of Klebsiella-targeting phages, clinical applications remain limited, with key phage-bacteria interactions still poorly understood. A major obstacle is fragmented access to well-characterised phage-bacteria pairings, restricting the collective advancement of therapeutic and mechanistic insights. To address this gap, we created the Klebsiella Phage Collection (KlebPhaCol), an open-source resource comprising 53 phages and 74 Klebsiella isolates, all fully characterised. These phages span five families - including a novel order, Felixvirales, associated with the human gut - and target 27 sequence types (including ST258, ST11, ST14) and 28 capsular-locus types (including KL1 and KL2), across six Klebsiella species. Freely accessible at www.klebphacol.org, KlebPhaCol invites the scientific community to both use and contribute to this resource, fostering collaborative research and a deeper understanding of Klebsiella-phage interactions beyond therapeutic use.

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