Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of post-surgical digestive fistula: towards an energetic nano-biotherapy
Mariani, A.; GUICHARD, A.; Sebbagh, A.; Cronemberger Andrade, A.; Al Amir Dache, Z.; Ribes, C.; Ayollo, D.; Karoui, M.; Lavieu, G.; Gazeau, F.; Silva, A. K. A.; Rahmi, G.; Mozafari, S.
Show abstract
BackgroundTissue regeneration heavily relies on cellular energy production, with mitochondria playing a crucial role. Dysfunctional mitochondria are implicated in various degenerative diseases, driving interest in targeting mitochondrial transplantation for tissue repair. Wound healing is highly compromised in gastrointestinal conditions resulting in fistula development, particularly after sleeve gastrectomy. Human mesenchymal stem/stromal cells (hMSCs) and their cell-free products such as mitochondria offer potential benefits due to their therapeutic properties on cellular energy production. Here we investigated the therapeutic advantage of hMSCs-derived mitochondria nano-biotherapy in a rat model of post-surgical fistula healing. MethodsViable and structurally intact mitochondria were isolated from hMSCs before exposure to human colonic epithelial cells (HCEC-1CT) culture or transplantation into a rat model of post-operative fistula. ResultsOur findings reveal significant dose-dependent improvement on cellular metabolic activity and ATP content of the recipient cells. Assessment of the external fistula orifice developed following post sleeve gastrectomy fistula, revealed a substantial healing in all transplanted rats compared to control group. ConclusionOur findings highlight the therapeutic potential of hMSCs-derived mitochondria in post-surgical fistula healing. This research contributes to advancing cell-free regenerative strategies for gastrointestinal conditions, offering new insights into mitochondrial-based therapies for enhancing wound healing and tissue repair.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells 94%
- Engineering of human myotubes toward a mature metabolic and contractile phenotype 93%
- Estrogen Deficiency alters Vascularization and Mineralization dynamics: insight from a novel 3D Humanized and Vascularized Bone Organoid Model 92%
Similar papers in this journal
- Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent 94%
- Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: a comparative analysis on the Umbilical Cord and Dental Pulp derived Stem/ Stromal Cells secretome 94%
- A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids 94%
Similar papers in this journal
- Development of novel apoptosis-assisted lung tissue decellularization methods 92%
- Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity 91%
- Antioxidant Response Activating nanoParticles (ARAPas) localize to atherosclerotic plaque and locally activate the Nrf2 pathway 91%
Similar papers in this journal
- Application and mechanism of anticancer peptides in organoid models of intrahepatic cholangiocarcinoma 94%
- SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation 94%
- Generation of Somatic Mitochondrial DNA-Replaced Cells for Mitochondrial Dysfunction Treatment 94%
Similar papers in this journal
- Pro-apoptotic and anti-invasive properties underscore the tumor suppressing impact of myoglobin on subset of human breast cancer cells 93%
- An optimized workflow for analyzing extracellular vesicles as biomarkers in liver diseases. 93%
- NDR2 Kinase Regulate Microglial Metabolic Adaptation and Inflammatory Response: Critical Role in Glucose-Dependent Functional Plasticity 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.