Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cells

The force-gated ion channel PIEZO1 confers mechanosensitivity to many cell types. While the structure and physiological roles of PIEZO1 are well-described, the subcellular distribution and the impact of the cellular microenvironment on PIEZO1 conformation and function are poorly understood. Here, using MINFLUX nanoscopy we demonstrate that PIEZO1 channels collectively deform the membrane into pit-shaped invaginations, thereby creating mechano-responsive microdomains capable of amplifying mechanical stimuli via subtle changes in their topology. Moreover, by measuring intramolecular distances in individual PIEZO1 channels with nanometer precision, we reveal subcellular compartment-specific differences in PIEZO1 conformation at rest and during activation that correlate with differences in PIEZO1 function and are possibly caused by differences in cytoskeletal architecture. Together, our data provide previously unrecognized insights into the complex interplay of forces that determine how PIEZO1 alters membrane shape and, vice versa, how the membrane together with the cytoskeleton affect the conformation and function of individual PIEZO1 channels. TeaserMINFLUX nanoscopy reveals subcellular distribution and conformational diversity of PIEZO1 channels in intact cells.