The Transcriptional Gradient in Negative-Strand RNA Viruses Suggests a Common RNA Transcription Mechanism
King, C. R.; Berezin, C.-T.; Munsky, B. E.; Peccoud, J.
Show abstract
We introduce a novel model of nonsegmented negative-strand RNA virus (NNSV) transcription. Previous models have relied on polymerase behavioral differences in the highly conserved intergenic sequences. Our model hypothesizes the transcriptional gradient in NNSVs is explained through a simple model with two parameters associated with the viral polymerase. Most differences in expression can be attributed to the processivity of the polymerase while additional attenuation occurs in the presence of overlapping genes. This model reveals a correlation between polymerase processivity and genome length, which is consistent with the universal entry of polymerases through the 3 end of the genome. Using this model, it is now possible to predict the transcriptional behavior of NNSVs from genotype alone, revolutionizing the design of novel NNSV variants for biomedical applications.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Indiscriminate activities of different Henipavirus polymerase complex proteins allow for efficient minigenome replication in hybrid systems 95%
- Characterization of Mollivirus kamchatka, the first modern representative of the proposed Molliviridae family of giant viruses 95%
- Murine norovirus mutants adapted to replicate in human cells reveal a post-entry restriction 95%
Similar papers in this journal
- Pervasive differential splicing in Marek’s Disease Virus can discriminate CVI-988 vaccine strain from RB-1B virulent strain in chicken embryonic fibroblasts 94%
- Single amino acid mutations effect Zika virus replication in vitro and virulence in vivo 94%
- Rotavirus A Genome Segments Show Distinct Segregation and Codon Usage Patterns 93%
Similar papers in this journal
- Direct RNA sequencing of Respiratory Syncytial Virus infected human cells generates a detailed overview of RSV polycistronic mRNA and transcript abundance 95%
- Increased elastase sensitivity and decreased intramolecular interactions in the more transmissible SARS-CoV-2 variants' spike protein 93%
- Machine learning using intrinsic genomic signatures for rapid classification of novel pathogens: COVID-19 case study 92%
Similar papers in this journal
- Genetic insertion of mouse Myxovirus-resistance gene 1 increases innate resistance against both high and low pathogenic avian influenza virus by significantly decreasing replication in chicken DF1 cell line 93%
- Evolution of resistance to fluoroquinolones by dengue virus serotype 4 provides insight into mechanism of action and consequences for viral fitness 93%
- Rolling Circle Amplification is a high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones. 93%
Similar papers in this journal
- Functional analysis of promoter element 2 within the viral polymerase gene of an emerging paramyxovirus, Sosuga virus 96%
- Widespread distribution and evolution of poxviral entry-fusion complex proteins in giant viruses 94%
- RNA-seq of the medusavirus suggests remodeling of the host nuclear environment at an early infection stage 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.