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A regulator controls both DNA damage response and anti-phage defense networks in Moraxellaceae

Song, S.; Zhong, S.; Shi, Q.; Zheng, X.; Yao, Y.; Wang, W.; Chen, S.; Huang, Z.; An, D.; Xu, H.; Tian, B.; Zhao, Y.; Wang, L.; Zhang, W.; Hua, X.; Yu, Y.; Lu, H.; Fan, L.; Hua, Y.

2024-11-11 microbiology
10.1101/2024.11.11.622897 bioRxiv
Show abstract

DNA damage chemicals, including many antibiotics, often induce prophage induction and phage outbreaks in microbial communities, a major threat to bacterial survival. Moraxellaceae contains clinically highly relevant strains with outstanding antibiotic and radio resistance, yet their cellular-level regulation in DNA damage response and anti-phage defense is largely unknown. Here, we identified a WYL family protein that had replaced the ubiquitous SOS system in evolution and directly regulated a transcriptional network of both DNA repair genes and anti-phage defense genes under DNA damage stress. This study depicts a mechanism that how bacteria maintain immunity to phages without compromising on antibiotic resistance, and sheds light on controlling the resistance of Moraxellaceae strains in clinical practice.

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