Understanding How Biopolymers Found in Marine Ecosystems Impact the Efficacy of Bacteriophages against Bacteria
Kwack, J.; Quan, D.; Kim, T.; Rupareliya, R. D.
Show abstract
Bacteriophages are abundant in Earths ecosystem and serve as valuable mediators in marine environments, regulating bacterial populations in oceans, hot springs, and salterns, among others. However, bacteriophages have high-specificity to their host, thus making it difficult to study bacteria-phage relationships. In this study, we observe whether naturally occurring biopolymers (coral mucin (from Stylophora pistillata), chitin, and sodium alginate) increase bacteriophage affinity to bacteria by recording the plaques formed throughout 15-minute time intervals. We use an isolated E. coli strain and its corresponding T7 phage, as well as isolated bacteria and phage strains from a coral sample (labeled Stylophora pistillata or "Coral 1"). The research conducted plaque assays using minimal media solution to quantify phage-forming units (PFUs) using 2 bacterial forms, Coral 1 and E. coli and Mucin, Sodium Alginate, and Chitin as biopolymers. Our research found that the biopolymers had an effective impact on the generation of PFUs in our bacterial cultures for all samples. While we were unable to conduct future plaque assays, the information we have now is important to discovering ways to increase phage efficiency in marine environments to help maintain nutrient resource levels in such ecosystems.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Effect of plastic and seaweed shelters on the skin microbiome of lumpfish Cyclopterus lumpus used as cleaner fish in aquaculture pens 94%
- Effect of perchlorate on biocementation capable bacteria and Martian bricks 94%
- Mesostats -- A multiplexed, low-cost, do-it-yourself continuous culturing system for experimental evolution of mesocosms 94%
Similar papers in this journal
- Separation of Bioactive Small Molecules, Peptide from Natural Products and Proteins from Pathogenic Microbe by Rotofor-based Preparative Isoelectric Focusing (IEF) Method 91%
- Mechanical Dissociation of Tissues for Single Cell Analysis Using a Simple Motorized Device 91%
- All-optical mechanobiology interrogation of YAP in human cancer and normal cells using a novel multi-functional system 90%
Similar papers in this journal
- Bacterial Zombies And Ghosts: Production Of Inactivated Gram-Positive And Gram-Negative Species With Preserved Cellular Morphology And Cytoplasmic Content 94%
- Microliter spotting and micro-colony observation: a rapid and simple approach for counting bacterial colony-forming units 94%
- Hyphal induction in Candida albicans: Optimizing medium parameters to accelerate hyphal growth enhanced by GlcNAc 92%
Similar papers in this journal
- A quick and reliable menthol-induced bleaching protocol for the Caribbean staghorn coral, Acropora cervicornis 94%
- Impact of NaCl, nitrate and temperature on the microbial community of a methanol-fed, denitrifying marine biofilm 93%
- Co-culturing Hyphomicrobium nitrativorans strain NL23 and Methylophaga nitratireducenticrescens strain JAM1 allows sustainable denitrifying activities under marine conditions 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.