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Optical Interference for the Guidance of Cryogenic Focused Ion Beam Milling Beyond the Axial Diffraction Limit

Sica, A. V.; Zaoralova, M.; Antolini, C.; Daan, B. B.; Penzes, J. J.; Malmqvist, L. M.; Jensen, G. J.; Jason, K. T.; Dahlberg, P.

2024-11-03 biophysics
10.1101/2024.11.01.621231 bioRxiv
Show abstract

Using a tri-coincident cryogenic FIB-SEM-LM system, we identify an interferometric optical response that can be used for targeting lamella production to fluorescently labelled structures with accuracy beyond the diffraction limit. We demonstrate the approach using synthetic samples of fluorescent beads embedded in micron-scale droplets of water. We then apply the approach to capture virions inside host cells. Successful targeting is confirmed by cryogenic electron tomography revealing clusters of virions in intracellular vesicles.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.