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An anterior hypothalamic circuit gates stress vulnerability

Pennington, Z. T.; LaBanca, A. R.; Abdel-Raheim, S. D.; Bacon, M. E.; Mahmoud, A. N.; Sompolpong, P.; Baggetta, A. M.; Zaki, Y.; Ko, B.; Dong, Z.; Smith, A. C.; Kenny, P. J.; Cai, D. J.

2024-10-28 neuroscience
10.1101/2024.10.28.620614 bioRxiv
Show abstract

Prior adversity increases susceptibility to subsequent stressful events, but the causal underlying changes in brain circuitry are poorly understood. We harnessed unbiased whole-brain activity mapping to identify circuits that are functionally remodeled by prior adversity. This revealed that the anterior hypothalamic nucleus (AHN) displays heightened stress reactivity in previously stressed mice. This was accompanied by increased functional connectivity between the AHN and a threat-related limbic network. Using in vivo Miniscope imaging, we found that neuronal activity in the AHN encodes stressor valence. Moreover, stimulating AHN neurons enhanced, and inhibiting their activity mitigated, reactivity to stressful events. Lastly, silencing amygdala inputs to the AHN abolished the ability of prior adversity to increase stress sensitivity. These findings define a key role of the AHN in gating stress vulnerability by scaling valence signals from the amygdala.

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