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Pipeasm: a tool for automated large chromosome-scale genome assembly and evaluation

Silva, B. M.; Trindade, F. d. J.; Canesin, L. E. C.; Souza, G. B. S.; Aleixo, A.; Nunes, G. L.; Oliveira, R. R. M.

2024-10-24 bioinformatics
10.1101/2024.10.21.598381 bioRxiv
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MotivationWith increasing initiatives to study biodiversity through high-quality reference genomes and the growing capacity of sequencing a wide range of organisms, there is a pressing need for an accessible, reproducible, and user-friendly tool that incorporates state-of-the-art methodologies for large genome assembly. ResultsWe introduce Pipeasm, a Snakemake pipeline designed for assembling vertebrate genomes using HiFi PacBio, ONT, and HiC data. By setting a configuration file with input information and suggested parameters, Pipeasm was able to assemble multiple-sized diploid genomes ready for manual curation. Availability and ImplementationPipeasm requires an environment with Snakemake and Singularity and is available at https://github.com/itvgenomics/pipeasm.

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