Back

Generation of cultured beef from bovine embryonic stem cells

Rui, X.; Li, Z.; Xu, J.; Dai, J.; Zhang, X.; Jin, X.; Liu, Y.

2024-10-18 bioengineering
10.1101/2024.10.15.618593 bioRxiv
Show abstract

Bovine embryonic stem cells (bESCs) serve as an optimal source for generating muscle and fat tissues. However, systematic methodologies for creating cultured beef from bESCs have not yet been reported. Here, we successfully established a bESC line and formulated a specialized culture medium that supports cell pluripotency in both two-dimensional (2D) and three-dimensional (3D) cultures. Additionally, we have also developed serum-free and transgene-free protocols that efficiently differentiate bESCs into muscle and fat cells. These differentiated cells were validated at the transcriptional and protein levels. Furthermore, we devised a method using high moisture stretched plant-based protein scaffolds for incorporating differentiated muscle and fat cells derived from bESCs, producing an innovative plant-cell hybrid cultured beef. These advancements provide a solid foundation for future cultured beef production using pluripotent stem cells.

Matching journals

The top 8 journals account for 50% of the predicted probability mass.

1
Stem Cell Research & Therapy
○ 30 papers in training set
Top 0.1%
18.8%
2
Biofabrication
○ 36 papers in training set
Top 0.1%
6.8%
3
Scientific Reports
○ 3612 papers in training set
Top 12%
6.4%
4
Advanced Science
○ 286 papers in training set
Top 1%
4.9%
5
Biotechnology and Bioengineering
○ 53 papers in training set
Top 0.2%
4.1%
6
Frontiers in Nutrition
○ 24 papers in training set
Top 0.2%
3.3%
7
Communications Biology
○ 993 papers in training set
Top 5%
3.3%
8
eLife
● 5828 papers in training set
Top 37%
2.8%
50% of probability mass above
9
ACS Synthetic Biology
● 287 papers in training set
Top 1.0%
2.8%
10
PLOS ONE
● 5266 papers in training set
Top 42%
2.4%
11
Biomaterials
○ 84 papers in training set
Top 0.7%
2.2%
12
Cytotherapy
○ 15 papers in training set
Top 0.1%
2.2%
13
Bioactive Materials
○ 20 papers in training set
Top 0.3%
2.0%
14
Advanced Healthcare Materials
○ 85 papers in training set
Top 0.9%
1.9%
15
Advanced Biology
○ 29 papers in training set
Top 0.2%
1.8%
16
The FASEB Journal
○ 194 papers in training set
Top 2%
1.8%
17
iScience
○ 1154 papers in training set
Top 20%
1.5%
18
Stem Cell Reports
○ 130 papers in training set
Top 1%
1.5%
19
Tissue Engineering Part A
○ 15 papers in training set
Top 0.2%
1.4%
20
Biotechnology Journal
○ 11 papers in training set
Top 0.1%
1.1%
21
Advanced Materials Interfaces
○ 10 papers in training set
Top 0.1%
1.1%
22
Frontiers in Bioengineering and Biotechnology
○ 98 papers in training set
Top 2%
1.0%
23
Annals of Biomedical Engineering
○ 37 papers in training set
Top 0.9%
1.0%
24
Science Advances
● 1243 papers in training set
Top 29%
0.9%
25
Biomaterials Advances
○ 22 papers in training set
Top 0.6%
0.9%
26
Journal of Biological Engineering
○ 12 papers in training set
Top 0.1%
0.9%
27
Small
○ 78 papers in training set
Top 1%
0.9%
28
Methods
○ 34 papers in training set
Top 0.6%
0.9%
29
Bioengineering
○ 29 papers in training set
Top 1%
0.9%
30
International Journal of Molecular Sciences
○ 494 papers in training set
Top 14%
0.9%